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prime script fast rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script fast rt reagent kit
    Prime Script Fast Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+fast+rt+reagent+kit/PrimeScript+FAST+RT+reagent+Kit+with+gDNA+Eraser/pm42010713-176-24-30
    Average 99 stars, based on 432 article reviews
    prime script fast rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Extraction:

    Article Title: Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis.
    Article Snippet: .. Total RNAs of testes and cells were extracted using the TRIZOL extraction method and tissue/cell total RNA extraction kit (Sparkjade, AC0202) and subsequently reversely transcribed into cDNA using Prime Script Fast RT reagent kit (TaKaRa, RR092A). .. The quantitative real-time PCR (qRT-PCR) mixture was prepared using TB Green Premix (TaKaRa, RR820A) and the primers listed in Supplementary Table S2.

    Article Title: Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis
    Article Snippet: .. Total RNAs of testes and cells were extracted using the TRIZOL extraction method and tissue/cell total RNA extraction kit (Sparkjade, AC0202) and subsequently reversely transcribed into cDNA using Prime Script Fast RT reagent kit (TaKaRa, RR092A). .. The quantitative real-time PCR (qRT-PCR) mixture was prepared using TB Green Premix (TaKaRa, RR820A) and the primers listed in Supplementary Table .

    RNA Extraction:

    Article Title: Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis.
    Article Snippet: .. Total RNAs of testes and cells were extracted using the TRIZOL extraction method and tissue/cell total RNA extraction kit (Sparkjade, AC0202) and subsequently reversely transcribed into cDNA using Prime Script Fast RT reagent kit (TaKaRa, RR092A). .. The quantitative real-time PCR (qRT-PCR) mixture was prepared using TB Green Premix (TaKaRa, RR820A) and the primers listed in Supplementary Table S2.

    Article Title: Gut microbiota induces dysspermatogenesis via microbial-derived phenylacetylglycine in Ggt1-deficient mice.
    Article Snippet: .. Total RNA of testes and cells were extracted using tissue/cell total RNA extraction kit (Shandong Sparkjade Biotechnology, AC0202), and reversely transcribed into cDNA using Prime Script Fast RT reagent kit (TaKaRa, RR092A). .. Triplicate quantitative real-time PCR (qRT-PCR) were employed using TB Green Premix (TaKaRa, RR820A).

    Article Title: Dnajc5b contributes to male fertility by maintaining the mitochondrial functions and autophagic homeostasis during spermiogenesis
    Article Snippet: .. Total RNAs of testes and cells were extracted using the TRIZOL extraction method and tissue/cell total RNA extraction kit (Sparkjade, AC0202) and subsequently reversely transcribed into cDNA using Prime Script Fast RT reagent kit (TaKaRa, RR092A). .. The quantitative real-time PCR (qRT-PCR) mixture was prepared using TB Green Premix (TaKaRa, RR820A) and the primers listed in Supplementary Table .

    Synthesized:

    Article Title: Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus.
    Article Snippet: .. The genomic DNA was then eliminated and firststrand cDNAs were synthesized using the Prime Script FAST RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s protocols. qRT-PCR was performed on a CFX Duet Real-time PCR System (Bio-Rad) using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). ..

    Article Title: Contribution of UbrA, a ubiquitin ligase essential for Arg/N-degron pathway, to peptidase gene expression in Aspergillus oryzae .
    Article Snippet: .. Complementary DNA was synthesized using the Prime Script FAST RT reagent kit with gDNA Eraser (Perfect Real Time) (Takara Bio Inc.). .. RT-qPCR analysis was performed using Mx3000P (Agilent Technologies Japan, Ltd., Tokyo, Japan).

    Article Title: ZmPOD5 positively regulates drought tolerance by modulating ROS production.
    Article Snippet: Drought stress is an important abiotic stress affecting maize (Zea mays L.) growth and productivity.. Class III peroxidases (PODs) are plant-specific enzymes that play crucial roles in plant growth, development, and responses to abiotic stress.. However, only a few studies have been conducted on the responses of PODs to drought stress in maize.

    Quantitative RT-PCR:

    Article Title: Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus.
    Article Snippet: .. The genomic DNA was then eliminated and firststrand cDNAs were synthesized using the Prime Script FAST RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s protocols. qRT-PCR was performed on a CFX Duet Real-time PCR System (Bio-Rad) using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Exploring the interaction between endornavirus and Sclerotinia sclerotiorum : mechanisms of phytopathogenic fungal virulence and antivirus.
    Article Snippet: .. The genomic DNA was then eliminated and firststrand cDNAs were synthesized using the Prime Script FAST RT Reagent Kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s protocols. qRT-PCR was performed on a CFX Duet Real-time PCR System (Bio-Rad) using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa, Dalian, China). ..



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